Comparative Histomorphologic ,Histomorphometric and Histochemistry Studies of Cecum in Adult Male and Female Black Local Turkeys of West Azarbaijan-Iran
Document Type : Original Article
Authors
Department of Basic Sciences, Faulty of Veterinary Medicine, Urmia University, Urmia, Iran
Abstract
Domestic fowl, including chickens, turkeys, and ducks, possess an organ known as the cecum, crucial for digestion and disease resistance due to its significant lymphoid tissue existence. However, limited information exists on the histology and histochemistry of the cecum in native breeds like the Azerbaijani Black Turkeys. This research delves into the histological examination of the cecal regions in male and female turkeys. For this purpose, to study the histology of the turkey cecum, 5 male and 5 female turkeys with an approximate weight of 4 to 6 kg were selected. After anesthetizing and euthanizing the animals, their ceca were separated and placed in a 10% formalin buffer solution. Tissue sections were prepared trough routine paraffin embedding technique and stained with hematoxylin-eosin for general examination and histomorphometry. Moreover, PAS staining was used to evaluate the dispersion of goblet cells in three parts of the male and female turkey cecum, and Masson's trichrome staining was applied for evaluating collagen bers. Results indicated anatomical similarities in cecal positioning among birds, with males showing greater mucosal layer thickness and lymph node size compared to females. Moreover, lymph node density varied between genders, with females exhibiting higher cell counts. This study provides valuable insights into the cecal structure of turkeys, shedding light on gender-specific histological differences and lymphoid cell distribution.
Keywords
[1] Al-Agele R, Jabbar AI, Ibrahim RS. Gross and
histological investigations on the cecum of the
black-crowned night heron (Nycticoarx nyctico-
rax). Syst Rev Pharm. 2020 Oct 1;11:1013-7.
[2] Bezuidenhout AJ. The spiral fold of the cae-
cum in the ostrich (Struthio camelus). Journal of
anatomy. 1993 Dec;183(Pt 3):587.
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related changes in the avian primary lymphoid or-
gans (thymus and bursa of Fabricius). Microscopy
research and technique. 2003 Dec 15;62(6):482-7.
[4] Erf GF. Cell-mediated immunity in poultry. Poul-
try science. 2004 Apr 1;83(4):580-90.
[5] Fathi H, Moghaddam G, Nematollahi A, Tah-
masbi A. The effect of macaroni waste on per-
formance of native turkeys in 3-8 weeks age. Ira-
nian Journal of Animal Science Research. 2009
Jun 22;1(2).
[6] Firth G. The normal lymphatic system of the
domestic fowl. Commonwealth Bureau of Animal
Health. 1977,47(3):167-179.
[7] Getty R, Sisson S, Grossman JD, Rosenbaum CE,
Ghoshal N, Hillman, D. Sisson and Grossman's
the Anatomy of the Domestic Animals, WB Saun-
ders company. 1975.
[8] Hassan SA, Moussa EA. Gross and microscopic
studies on the stomach of domestic duck (Anas
platyrhynchos) and domestic pigeon (Columba
livia domestica). Journal of Veterinary Anatomy.
2012 Oct 1;5(2):105-27.
[9] Hussein AA, Reshag AF. Immunehistochemical
and histomorphological study of postnatal devel-
opment of the cecal tonsils in Turkey (Melea-
gris Gallopavo). The Iraqi Journal of Veterinary
Medicine. 2019 Aug 4;43(1):85-92.
[10] Potter MA, Lentle RG, Minson CJ, Birtles MJ,
Thomas D, Hendriks WH. Gastrointestinal tract
of the brown kiwi (Apteryx mantelli). Journal of
Zoology. 2006 Nov;270(3):429-36.
[11] Pourrhaji MJ, Hashemi SR. Anatomical, morpho-
metrical and histological study of large intestine
in muscovy duck (Cairina moschata). Quarterly
Scienti c Research Journal of Animal Physiology
and Development, 12(3):79-90.
[12] Rezaian M, Hamedi S. Histological study of the
caecal tonsil in the cecum of 4-6 months old white
leghorn chicks. American Journal of Animal and
Veterinary Sciences, 2007, 2(2):50-54.
Comparative Histomorphologic ,Histomorphometric and Histochemistry Studies of Cecum in Adult Male and
Female Black Local Turkeys of West Azarbaijan-Iran: 27{37 36
[13] Turk DE. Symposium: The avian gastroin-
testinal tract and digestion. Poultry Science.
1982;61:1225-44.
[14] Warui CN, Erlwanger KH, Skadhauge E. Gross
anatomical and histomorphological observations
on the terminal rectum and the cloaca in the
Ostrich Struthio camelus. Ostrich. 2009 Dec
1;80(3):185-91.
histological investigations on the cecum of the
black-crowned night heron (Nycticoarx nyctico-
rax). Syst Rev Pharm. 2020 Oct 1;11:1013-7.
[2] Bezuidenhout AJ. The spiral fold of the cae-
cum in the ostrich (Struthio camelus). Journal of
anatomy. 1993 Dec;183(Pt 3):587.
[3] Ciriaco E, P~nera PP, Daz-Esnal B, Laura R. Age-
related changes in the avian primary lymphoid or-
gans (thymus and bursa of Fabricius). Microscopy
research and technique. 2003 Dec 15;62(6):482-7.
[4] Erf GF. Cell-mediated immunity in poultry. Poul-
try science. 2004 Apr 1;83(4):580-90.
[5] Fathi H, Moghaddam G, Nematollahi A, Tah-
masbi A. The effect of macaroni waste on per-
formance of native turkeys in 3-8 weeks age. Ira-
nian Journal of Animal Science Research. 2009
Jun 22;1(2).
[6] Firth G. The normal lymphatic system of the
domestic fowl. Commonwealth Bureau of Animal
Health. 1977,47(3):167-179.
[7] Getty R, Sisson S, Grossman JD, Rosenbaum CE,
Ghoshal N, Hillman, D. Sisson and Grossman's
the Anatomy of the Domestic Animals, WB Saun-
ders company. 1975.
[8] Hassan SA, Moussa EA. Gross and microscopic
studies on the stomach of domestic duck (Anas
platyrhynchos) and domestic pigeon (Columba
livia domestica). Journal of Veterinary Anatomy.
2012 Oct 1;5(2):105-27.
[9] Hussein AA, Reshag AF. Immunehistochemical
and histomorphological study of postnatal devel-
opment of the cecal tonsils in Turkey (Melea-
gris Gallopavo). The Iraqi Journal of Veterinary
Medicine. 2019 Aug 4;43(1):85-92.
[10] Potter MA, Lentle RG, Minson CJ, Birtles MJ,
Thomas D, Hendriks WH. Gastrointestinal tract
of the brown kiwi (Apteryx mantelli). Journal of
Zoology. 2006 Nov;270(3):429-36.
[11] Pourrhaji MJ, Hashemi SR. Anatomical, morpho-
metrical and histological study of large intestine
in muscovy duck (Cairina moschata). Quarterly
Scienti c Research Journal of Animal Physiology
and Development, 12(3):79-90.
[12] Rezaian M, Hamedi S. Histological study of the
caecal tonsil in the cecum of 4-6 months old white
leghorn chicks. American Journal of Animal and
Veterinary Sciences, 2007, 2(2):50-54.
Comparative Histomorphologic ,Histomorphometric and Histochemistry Studies of Cecum in Adult Male and
Female Black Local Turkeys of West Azarbaijan-Iran: 27{37 36
[13] Turk DE. Symposium: The avian gastroin-
testinal tract and digestion. Poultry Science.
1982;61:1225-44.
[14] Warui CN, Erlwanger KH, Skadhauge E. Gross
anatomical and histomorphological observations
on the terminal rectum and the cloaca in the
Ostrich Struthio camelus. Ostrich. 2009 Dec
1;80(3):185-91.
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