Histological Study of Ilam Native Hens Reproductive System
Document Type : Original Article
Authors
Department of Histology, Faculty of Veterinary Medicine, Ilam University, Ilam, Iran
10.22084/avr.2025.30716.1002
Abstract
Considering the importance of the chicken female reproductive system in egg production and reproduction and production of chickens as well as the development of breeding to preserve six pieces of chicken the native species of Ilam, its histological study is necessary to know more about this system and provide a basis for histological studies. In this study, native chickens of Ilam were prepared, ovaries and oviduct samples xed and studied after H&E staining. Results showed tonica albugina, a thin layer of dense connective tissue, containing the ovarian capsule. The ovarian surface epithelium appears as Simple columnar epithelium, cubic or short columnar cells. The follicles are surrounded by a layer of granulosa cells around each follicle. The magnum is covered by anastomotic protrusions and mucosal folds. The tubular glands completely close in the mucosal layer in each crease. The super cial epithelium of the isthmus is of the ciliated, secretory-like cylindrical type. The short tubular glands and the epithelium are simple cubic glands. The lining of the uterus is a quasi-layered cylindrical type of eyelashes. Mucosa-submucosa has ruptured tubular glands. The muscular layer, especially the annular layer of the vagina, is well developed and the surface of the vagina is covered with mucous-like cylindrical epithelium-like cylindrical epithelium. Tubular glands are located inside the vaginal mucosal connective tissue near the junction with the uterus. In general, there are very close similarities between the reproductive tissues of the native chicken of Ilam and other chickens.
Keywords
[1] Eurell JA, Frappier BL, editors. Dellmann's text-
book of veterinary histology. John Wiley & Sons;
2013 Mar 19.
[2] Galloway SM, McNatty KP, Cambridge LM, Laiti-
nen MP, Juengel JL, Jokiranta TS, McLaren RJ,
Luiro K, Dodds KG, Montgomery GW, Beattie
AE. Mutations in an oocyte-derived growth fac-
tor gene (BMP15) cause increased ovulation rate
and infertility in a dosage-sensitive manner. Na-
ture genetics. 2000 Jul;25(3):279-83.
[3] Giles JR, Elkin RG, Trevino LS, Urick ME, Ra-
machandran R, Johnson PA. The restricted ovu-
lator chicken: a unique animal model for inves-
tigating the etiology of ovarian cancer. Interna-
tional Journal of Gynecological Cancer. 2010 Jun
1;20(5):738-44.
[4] Kalantari-Hesari A, Morovvati H, Babaei M,
Nourian A, Esfandiari K, Elmi T, Soltani S.
Modi ed methods to simpli cation histochemi-
cal, immunohistochemical, and hematoxylin-eosin
staining. Iranian Veterinary Journal. 2022 Nov
22;18(3):63-73.
[5] Klomp NI, Furness RW. Non-breeders as a buffer
against environmental stress: declines in numbers
of great skuas on Foula, Shetland, and prediction
of future recruitment. Journal of Applied Ecology.
1992 Jan 1:341-8.
[6] Mohammadpour AA, Zamanimoghadam A, Hei-
dari M. Comparative histomorphometrical study
of genital tract in adult laying hen and duck. In-
Veterinary Research Forum 2012, 3(1):27.
[7] Sari ML, Noor RR, Hardjosworo PS, Nisa C.
Characteristics Morphology female reproductive
system pegagan ducks. International Journal of
Chemical Engineering and Applications. 2014 Aug
1;5(4):307.
[8] O'Shannessy DJ, Jackson SM, Twine NC, Hoff-
man BE, Dezso Z, Agoulnik SI, Somers EB. Gene
expression analyses support fallopian tube epithe-
lium as the cell of origin of epithelial ovarian can-
cer. International journal of molecular sciences.
2013 Jul 1;14(7):13687-703.
[9] Perche O, Hayashi M, Hayashi K, Birk D, Trel-
stad RL, Sandoz D. Origin of type I collagen lo-
calized within oviduct epithelium of quail hyper-
stimulated by progesterone. Journal of Cell Sci-
ence. 1990 Jan 1;95(1):85-95.
[10] Pyle P, Spear LB, Sydeman WJ, Ainley DG. The
effects of experience and age on the breeding pe-
formance of Western Gulls. The Auk. 1991 Jan
1:25-33.
[11] Rezaian, Maryam. Poultry histology. 2013. Uni-
versity of Tehran Press.
[12] Shahrooz, Rasool. Delman Veterinary Histology
(Persian translation). Young author Everell and
Brian L. Frappier. Urmia University Press. 1392.
119, 541-546.
[13] Sultana F, Yokoe A, Ito Y, Mao KM, Yoshizaki N.
The peri-albumen layer: a novel structure in the
envelopes of an avian egg. Journal of Anatomy.
2003 Jul;203(1):115-22.
[14] Trevi~no LS, Giles JR, Wang W, Urick ME, John-
son PA. Gene expression pro ling reveals differ-
entially expressed genes in ovarian cancer of the
hen: support for oviductal origin?. Hormones and
Cancer. 2010 Aug;1(4):177-86.
[15] Wani H, et al. Color atlas of veterinary histology.
2nd ed. Printed in the United States of America.
2017, 2000.
[16] Nugrahini YL, Pradipta MS. Reproduction Per-
formance of Magelang Duck Evaluated by Histo-
logical Preparation. Journal of Livestock Science
and Production. 2018 Nov 9;2(2):99-102.
book of veterinary histology. John Wiley & Sons;
2013 Mar 19.
[2] Galloway SM, McNatty KP, Cambridge LM, Laiti-
nen MP, Juengel JL, Jokiranta TS, McLaren RJ,
Luiro K, Dodds KG, Montgomery GW, Beattie
AE. Mutations in an oocyte-derived growth fac-
tor gene (BMP15) cause increased ovulation rate
and infertility in a dosage-sensitive manner. Na-
ture genetics. 2000 Jul;25(3):279-83.
[3] Giles JR, Elkin RG, Trevino LS, Urick ME, Ra-
machandran R, Johnson PA. The restricted ovu-
lator chicken: a unique animal model for inves-
tigating the etiology of ovarian cancer. Interna-
tional Journal of Gynecological Cancer. 2010 Jun
1;20(5):738-44.
[4] Kalantari-Hesari A, Morovvati H, Babaei M,
Nourian A, Esfandiari K, Elmi T, Soltani S.
Modi ed methods to simpli cation histochemi-
cal, immunohistochemical, and hematoxylin-eosin
staining. Iranian Veterinary Journal. 2022 Nov
22;18(3):63-73.
[5] Klomp NI, Furness RW. Non-breeders as a buffer
against environmental stress: declines in numbers
of great skuas on Foula, Shetland, and prediction
of future recruitment. Journal of Applied Ecology.
1992 Jan 1:341-8.
[6] Mohammadpour AA, Zamanimoghadam A, Hei-
dari M. Comparative histomorphometrical study
of genital tract in adult laying hen and duck. In-
Veterinary Research Forum 2012, 3(1):27.
[7] Sari ML, Noor RR, Hardjosworo PS, Nisa C.
Characteristics Morphology female reproductive
system pegagan ducks. International Journal of
Chemical Engineering and Applications. 2014 Aug
1;5(4):307.
[8] O'Shannessy DJ, Jackson SM, Twine NC, Hoff-
man BE, Dezso Z, Agoulnik SI, Somers EB. Gene
expression analyses support fallopian tube epithe-
lium as the cell of origin of epithelial ovarian can-
cer. International journal of molecular sciences.
2013 Jul 1;14(7):13687-703.
[9] Perche O, Hayashi M, Hayashi K, Birk D, Trel-
stad RL, Sandoz D. Origin of type I collagen lo-
calized within oviduct epithelium of quail hyper-
stimulated by progesterone. Journal of Cell Sci-
ence. 1990 Jan 1;95(1):85-95.
[10] Pyle P, Spear LB, Sydeman WJ, Ainley DG. The
effects of experience and age on the breeding pe-
formance of Western Gulls. The Auk. 1991 Jan
1:25-33.
[11] Rezaian, Maryam. Poultry histology. 2013. Uni-
versity of Tehran Press.
[12] Shahrooz, Rasool. Delman Veterinary Histology
(Persian translation). Young author Everell and
Brian L. Frappier. Urmia University Press. 1392.
119, 541-546.
[13] Sultana F, Yokoe A, Ito Y, Mao KM, Yoshizaki N.
The peri-albumen layer: a novel structure in the
envelopes of an avian egg. Journal of Anatomy.
2003 Jul;203(1):115-22.
[14] Trevi~no LS, Giles JR, Wang W, Urick ME, John-
son PA. Gene expression pro ling reveals differ-
entially expressed genes in ovarian cancer of the
hen: support for oviductal origin?. Hormones and
Cancer. 2010 Aug;1(4):177-86.
[15] Wani H, et al. Color atlas of veterinary histology.
2nd ed. Printed in the United States of America.
2017, 2000.
[16] Nugrahini YL, Pradipta MS. Reproduction Per-
formance of Magelang Duck Evaluated by Histo-
logical Preparation. Journal of Livestock Science
and Production. 2018 Nov 9;2(2):99-102.
)