Molecular Detection of Mycoplasma in Milk Samples of Goat Herds in Borujerd County
Document Type : Original Article
Authors
1 Department of Pathobiology, Faculty of Veterinary Medicine, Bu-Ali Sina University, Hamedan, Iran,
2 Department of Pathobiology, Faculty of Veterinary Medicine, Bu-Ali Sina University, Hamedan, Iran
3 Department of Clinical Sciences, Faculty of Veterinary Medicine, Bu-Ali Sina University, Hamedan, Iran
Abstract
Mycoplasmas are among the smallest bacteria which are responsible for economically signi cant infections in small ruminants. This study aimed to detect Mycoplasma in goat milk samples from Borujerd County, Iran. Ninety-six milk samples were collected from seven regions and screened for subclinical mastitis using the California Mastitis Test (CMT) and somatic cell count (SCC). Thereafter, total DNA was extracted, and Mycoplasma detection was performed by a PCR assay using genus-speci c primers. High SCC and CMT scores were observed in 7.3% and 31.25% of samples, respectively. PCR analysis con rmed Mycoplasma infection in 5.2% (n=5) of the milk samples. The presence of Mycoplasma infection in goat herds in Borujerd county highlights the need for breeders and veterinary supervisors to take effective measures to control and prevent these infections. Besides, these ndings suggest PCR as say as a rapid and reliable method for identifying Mycoplasma in milk samples.
Keywords
References
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coplasma agalactiae by culture and polymerase
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Detection of Mycoplasma agalactiae from Semen
Samples of Goats. Archives of Razi Institute.
2017;72(3):159-164.
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Kheirkhah B, Abtin AR, Ashtari A Shamsad-
dini Bafti M. Comparison of infection rate of My-
coplasma agalactiae in affected sheep with goats to
contagious agalactia in Kerman province. Journal
of Comparative Pathobiology. 2013; 1001-1006.
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Sayehban P. Isolation and Identi cation of My-
coplasma agalactiae by Culture and Polymerase
Chain Reaction Methods in the Sheep Herds in
Guilan Province, Iran. Archives of Razi Institute.
2017;72(4):219-223.
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dairy science. 2017;100(12):10381-10397.
[19] Shamsaddini Bafti M, Pourbakhsh SA, Eza-
tkhah M, & Ashtari A. Detection of Mycoplasma
agalactiae in Small Ruminants of Southeast Iran.
Archives of Razi Institute. 2017;72(4):237-242.
Avicenna Veterinary Research/ Vol. 1, No. 3, Summer 2025 43
[20] Tatay-Dualde J, Sanchez A, Prats-van der Ham
M, A., Gomez-Martn M, Paterna A, Corrales
J, De la Fe C, Contreras A, & Amores J. Sen-
sitivity of two methods to detect Mycoplasma
agalactiae in goat milk. Irish veterinary journal.
2015;68(1):21.
[21] Templeton KE, Scheltinga SA, Graffelman AW,
Van Schie JM, Crielaard JW, Sillekens P, Van
den Broek PJ, Goossens H, Beersma MF, & Claas
EC. Comparison and evaluation of real-time PCR,
real-time nucleic acid sequence-based ampli ca-
tion, conventional PCR, and serology for diagno-
sis of Mycoplasma pneumoniae. Journal of clinical
microbiology. 2003:41(9):4366-4371.
[22] Zamiri M, & Heidari A. Reproductive character-
istics of Rayini male goats of Kerman province
in Iran. Animal reproduction science. 2006;96(1-
2):176-185.
[1] Contreras A, Sanchez D, Corrales A, Marco J,
Paape M, & Gonzalo C. Mastitis in small ru-
minants. Small Ruminant Research. 2007;68(1-
2):145-153.
[2] De la Fe C, Amores J, Martn AG, Sanchez A,
Contreras A, & Corrales J. Mycoplasma agalac-
tiae detected in the semen of goat bucks. Theri-
ogenology. 2009;72(9):1278-1281.
[3] Dohoo IR, Meek A, Meek A, & Martin S. So-
matic cell counts in bovine milk. Canadian Jour-
nal of Comparative Medicine. Revue Canadienne
de Medecine Comparee. 1984;48(2):130-135.
[4] Hajizadeh A, Ghaderi R, Ayling RD. Species of
Mycoplasma causing contagious agalactia in small
ruminants in Northwest Iran. Veterinaria italiana.
2018;54(3):205-210.
[5] Jay M, & Tardy F. Contagious agalactia in
sheep and goats: Current perspectives. Veterinary
Medicine: Research and Reports. 2019;10:229-247.
[6] Kumar A, Rahal A, Chakraborty S, Verma AK,
& Dhama K. Mycoplasma agalactiae, an etiolog-
ical agent of contagious agalactia in small ru-
minants: a review. Veterinary medicine interna-
tional. 2014;1(12):1-13.
[7] Lierz M, Hagen N, Harcourt-Brown N, Hernandez-
Divers SJ, Luschow D, & Hafez HM. Prevalence of
mycoplasmas in eggs from birds of prey using cul-
ture and a genus-speci c mycoplasma polymerase
chain reaction. Avian Pathology. 2007;36(2):145-
150.
[8] Madanat A, Zendulkova D. & Pospsil Z. Conta-
gious agalactia of sheep and goats. A review. Acta
Veterinaria Brno. 2001;70(4):403-412.
[9] McGowin CL, & Totten PA. The unique microbi-
ology and molecular pathogenesis of Mycoplasma
genitalium. The Journal of infectious diseases.
2017;(suppl 2):S382-S388.
[10] Mohammadpour M, Pourbakhsh SA, Abtin AR,
Naderi H. Isolation and identi cation of My-
coplasma agalactiae by culture and polymerase
chain reaction (PCR) from milk of sheep in Masal,
Guilan. New Cellular and Biotechnology Journal.
2015;5(18):49-54.
[11] Moradi Bidhendi S, Khaki P, & Pilehchian R. Iso-
lation and identi cation of Mycoplasma agalac-
tiae by culture and Polymerase Chain Reac-
tion in Sheep and Goat Milk Samples in Ko-
rdestan province, Iran. Archives of Razi Institute.
2011;66(1):11-16.
[12] Persson Y, & Olofsson I. Direct and indirect mea-
surement of somatic cell count as indicator of in-
tramammary infection in dairy goats. Acta Vet-
erinaria Scandinavica. 2011;53(1):15.
[13] Pourbakhsh S, Abtin A, Ashtari A, Kheirkhah
B, Bayatzadeh M, & Ahangran S. Isolation and
Detection of Mycoplasma agalactiae from Semen
Samples of Goats. Archives of Razi Institute.
2017;72(3):159-164.
[14] Pourbakhsh SA, Hamidavi Mohammadpour S,
Kheirkhah B, Abtin AR, Ashtari A Shamsad-
dini Bafti M. Comparison of infection rate of My-
coplasma agalactiae in affected sheep with goats to
contagious agalactia in Kerman province. Journal
of Comparative Pathobiology. 2013; 1001-1006.
[15] Quinn PJ, Markey BK, Leonard FC, Fitzpatrick
Ei, Fanning S, & Hartigan P. Veterinary micro-
biology and microbial disease. 2nd edition, John
Wiley & Sons. 2011;pp:1000-1024.
[16] Quinn PJ, Markey BK, Leonard F, FitzPatrick E,
& Fanning S. Concise review of veterinary micro-
biology. John Wiley & Sons. 2015;pp:88-91.
[17] Rahimabadi E, Asadpour Y, Pourbakhsh SA, &
Sayehban P. Isolation and Identi cation of My-
coplasma agalactiae by Culture and Polymerase
Chain Reaction Methods in the Sheep Herds in
Guilan Province, Iran. Archives of Razi Institute.
2017;72(4):219-223.
[18] Ruegg PL. A 100-Year Review: Mastitis detec-
tion, management, and prevention. Journal of
dairy science. 2017;100(12):10381-10397.
[19] Shamsaddini Bafti M, Pourbakhsh SA, Eza-
tkhah M, & Ashtari A. Detection of Mycoplasma
agalactiae in Small Ruminants of Southeast Iran.
Archives of Razi Institute. 2017;72(4):237-242.
Avicenna Veterinary Research/ Vol. 1, No. 3, Summer 2025 43
[20] Tatay-Dualde J, Sanchez A, Prats-van der Ham
M, A., Gomez-Martn M, Paterna A, Corrales
J, De la Fe C, Contreras A, & Amores J. Sen-
sitivity of two methods to detect Mycoplasma
agalactiae in goat milk. Irish veterinary journal.
2015;68(1):21.
[21] Templeton KE, Scheltinga SA, Graffelman AW,
Van Schie JM, Crielaard JW, Sillekens P, Van
den Broek PJ, Goossens H, Beersma MF, & Claas
EC. Comparison and evaluation of real-time PCR,
real-time nucleic acid sequence-based ampli ca-
tion, conventional PCR, and serology for diagno-
sis of Mycoplasma pneumoniae. Journal of clinical
microbiology. 2003:41(9):4366-4371.
[22] Zamiri M, & Heidari A. Reproductive character-
istics of Rayini male goats of Kerman province
in Iran. Animal reproduction science. 2006;96(1-
2):176-185.
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