Study of the Histological Structure of Hardin's Glands in Khalkhali Goats
Document Type : Original Article
Authors
1 Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
2 Department of Basic Sciences, Faculty of Veterinary Medicine, Bu-Ali Sina University, Hamedan, Iran
Abstract
The Khalkhali goat is a black dairy breed found in the Azerbaijan region. The Harderian gland is only found in some species. These glands secrete an oily substance rich in porphyrin that facilitates the movement of the eyelids over the eye and creates an immune response. So far, no study has been conducted on the histological structure of the Hardin glands in the eyes of Khalkhali goats. In this study, 4-goats slaughtered at the Zanjan slaughterhouse were used. After, the heads of the goats were separated from the trunk and the eyelids along with the Hardin glands were separated from the skull bone. The samples were placed in 10% formalin. The sample was xated and entered the tissue passage and after preparing paraffin blocks, 5-um thick sections were prepared and stained using the hematoxylin-eosin method. Histological examination showed that this gland in the Khalkhali goat is covered from all sides by a capsule of tissue. Blades of the capsule enter the parenchyma of the gland and divide it into lobules. The lobules of this gland include serous, mucous, and seromucous secretory units, and the secretions of these units are collected by intralobular ducts. It can be concluded that the Harderian gland in the Khalkhali goat breed was similar to the Harderian glands of the alpaca in terms of the type of secretions of the acini units and in terms of the interlobular and extralobular ducts, it was very similar to the existing reports on cattle, other goat breeds, and sheep.
Keywords
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study of dorsal lacrimal gland in Iranian river buf-
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Study of the Histological Structure of Hardin's Glands in Khalkhali Goats: 29{33 32
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histochemical study of the lacrimal apparatus of
sheep and goats. American Journal of Veterinary
Research. 1966 Nov;27(121):1633-40.
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mestic animals WD Saunders Company. Philadel-
phia, USA. 1940:228-1745.
skeleton of the eye: I. Conjunctival papillae and
scleral ossicles. Developmental biology. 1962 Dec
1;5(3):382-401.
[2] Gargiulo AM, Coliolo P, Ceccarelli P, Pedini V.
Ultrastructural study of sheep lacrimal glands.
Veterinary research. 1999;30(4):345-51.
[3] Gargiulo AM, Dall'Aglio C, Coliolo P, Ceccarelli
P, Pedini V. Complex carbohydrate histochem-
istry and ultracytochemistry of the sheep lacrimal
gland. Anatomia, Histologia, Embryologia. 2000
Feb;29(1):19-24.
[4] Gelatt KN, Brooks DE, Kallberg ME. The canine
glaucomas. Essentials of veterinary ophthalmol-
ogy. 2008 Apr 22;2:155-87.
[5] Kassab A, El-Zoghby I. Anatomical and histolog-
ical studies of the aqueous out ow system in the
eye of goat (Capra hircus). Journal of Veterinary
Anatomy. 2010 Oct 1;3(2):13-22.
[6] Kleckowska-Nawrot J, Marycz K, Czoga la J, Ku-
jawa K, Janeczek M, Chroszcz A, Brudnicki W.
Morphology of the Lacrimal Gland and Super cial
Gland of the Third Eyelid of Roe Deer (Capreolus
Capreolus L.). Pakistan Veterinary Journal. 2013
Apr 1;33(2).
[7] Kleckowska-Nawrot J, Nowaczyk R, Gozdziewska-
Har lajczuk K, Krasucki K, Janeczek M. Histologi-
cal, histochemical and ne structure studies of the
lacrimal gland and super cial gland of the third
eyelid and their signi cance on the proper func-
tion of the eyeball in alpaca (Vicugna pacos). Folia
morphologica. 2015;74(2):195-205.
[8] Kuhnel W. Vergleichende histologische, histo-
chemische und elektronenmikroskopische Unter-
suchungen an Tranendrusen. Zeitschrift fur Zell-
forschung und Mikroskopische Anatomie. 1968
Dec;89(4):550-72.
[9] Miedel EL, Hankenson FC. Biology and diseases
of hamsters. InLaboratory animal medicine 2015
Jan 1 (pp. 209-245). Academic Press. 209-245.
[10] Morovvati, H., Kalantari-Hesari, A. (2019) Text
book of histotechniques and lab management. Uni-
versity of Tehran Press. First Edition.
[11] Nasirian, A., Pashmi, M., Haji Seyed javadi,
M.M., Nouri, M., Haji Seyed javadi, M.A., Egh-
bali, M., Abbasi, M. (2009). Atlas of livestock
breeds (cattle, sheep, goats and horses) of Iran
and the world. Srva Press.
[12] Olopade JO, Kwari HD, Agbashe IO, Onwuka SK.
Morphometric study of the eyeball of three breeds
of goats in Nigeria. Int J Morphol. 2005;23(4):377-
80.
[13] Rajathi S, Ramesh G, Raja K, Kannan TA, Sri-
ram P, Hemalatha S. Microscopic anatomy of
harderian gland in goats. J. Entomol. Zool. Stud.
2019;7:1413-8.
[14] Rana T, editor. Principles of Goat Disease and
Prevention. John Wiley & Sons; 2023 Aug 15.
[15] Schlegel T, Brehm H, Amselgruber WM. The car-
tilage of the third eyelid: a comparative macro-
scopical and histological study in domestic ani-
mals. Annals of Anatomy-Anatomischer Anzeiger.
2001 Mar 1;183(2):165-9.
[16] Shadkhast M, Bigham AS. A Histo-Anatomical
study of dorsal lacrimal gland in Iranian river buf-
falo. Vet Scanj Online Veterinary Medical Journal.
2010 Dec 31;5(1).
Study of the Histological Structure of Hardin's Glands in Khalkhali Goats: 29{33 32
[17] Sinha RD, Calhoun ML. A gross, histologic, and
histochemical study of the lacrimal apparatus of
sheep and goats. American Journal of Veterinary
Research. 1966 Nov;27(121):1633-40.
[18] Sisson S, Grossman JD. The anatomy of the do-
mestic animals WD Saunders Company. Philadel-
phia, USA. 1940:228-1745.
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